Analyzing PFAS and Androgens through HPLC and Radiochromatography

Unraveling the Mysteries of PFAS and Androgens

In a recent interview for the Concentrating on Chromatography series, David Oliva spoke with Andrea Andress Huacachino, a PhD student at the University of Pennsylvania working in Dr. Trevor Penning's lab. Andrea's research focuses on the intricate world of biochemistry, biophysics, and toxicology, with a particular emphasis on the impact of environmental pollutants like PFAS on hormonal regulation.

The PFAS-Androgen Connection

Andrea's work delves into the complex relationship between per- and polyfluoroalkyl substances (PFAS) and androgens, a class of hormones often associated with male characteristics but present in all humans. Her research has uncovered a novel finding regarding perfluorooctanoic acid (PFOA), a type of PFAS:

- PFOA affects androgen regulation at the pre-receptor level
- It inhibits the enzyme aldo-reductase 1C2, which normally inactivates potent androgens
- This inhibition leads to higher levels of active androgens in the body

Innovative Analytical Techniques

To conduct her groundbreaking research, Andrea employs a unique combination of analytical techniques:

Radio Chromatography: A method that combines high-performance liquid chromatography (HPLC) with radioactive detection. This technique allows for the separation and quantification of androgens and their metabolites with minimal sample manipulation.

Sample Preparation: 
1. Extract androgens from cell media using diethyl ether
2. Dry down the organic layer using nitrogen
3. Reconstitute in 60% acetonitrile for HPLC analysis

Challenges and Triumphs

Andrea's work is not without its challenges. The structural similarities between androgens make separation difficult, requiring extensive method development and optimization. However, these challenges also provide opportunities for innovation and discovery.

The Bigger Picture

Andrea emphasizes the importance of understanding androgen regulation:

- Androgens affect everyone, regardless of sex or gender
- Environmental factors can significantly impact androgen levels
- Dysregulation of androgens can lead to various health issues, including polycystic ovarian syndrome and prostate cancer

As Andrea continues her research, she contributes valuable insights to the fields of toxicology and endocrinology. Her work not only advances our understanding of PFAS and their effects on human health but also showcases the power of analytical chemistry in solving complex biological puzzles.

For those interested in learning more about Andrea's work or connecting with her professionally, you can find her on LinkedIn at https://www.linkedin.com/in/andrea-andress-huacachino-9193a7154/